1. In Vivo Hepatic Reprogramming of disease-associated cell types into fully functional hepatocytes as a possible therapy for end-stage liver disease/liver cirrhosis. We work with “humanized” mice harboring human liver cells (mainly liver stellate cells and hepatocytes) and perform in vivo screens to determine via positive selection those gene combinations that regenerate livers most efficiently.
2. Genetic screen in human liver organoids made from adult tissue stem cells and iPSCs. In this project, we perform a screen in liver organoids. The screen will reveal genetic and epigenetic drivers of hepatocyte and biliary regeneration. Further, multi-lineage organoids will elucidate the multi-lineage crosstalk in liver regeneration. We validate the findings in engraftment studies in vivo.